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Deep Dive explores ‘Reefs, Wrecks, and Rascals’

Arthur Dunkelman, curator of the University of Miami Kislak Collection, shared his passion and expertise in a webinar...
Honoring the founding women of the Cuban Heritage Collection

Without their foresight and love of homeland, the collection—the largest repository of Cuban documents outside of the...

Theses & Dissertations (Scholarship@Miami)

Human immunoglobulin lambda light chain genes and lambda-like genes: Identification and characterization of a new C lambda gene and chromosomal localization and expression of human lambda-like genes in pre-B lymphocytes

The characterization and expression of human immunoglobulin lambda ($\lambda$) and lambda-like genes are presented. The human immunoglobulin lambda gene locus had been previously described as containing six JC genes, with genes JC$\lambda$1, 2, 3, and 6 encoding the Mcg, Kern$\sp-$Oz$\sp-$, Kern$\sp-$Oz$\sp+$, and Kern$\sp+$Oz$\sp-$ myeloma proteins.In contrast, we determined JC$\lambda$6 was a pseudogene by DNA subcloning of the gene and Southern blots of 32 different genomic DNA samples. JC$\lambda$6 was found to contain a four base-pair insertion creating an inframe TAG termination codon in the C region.We further identified a new $\lambda$ gene, JC$\lambda$7, found downstream of C$\lambda$6. DNA sequencing of the JC$\lambda$ regions of $\lambda$7 revealed it was a potential functional gene since it contained consensus recombination signals, RNA splice sites and maintained open reading frames throughout its coding regions. We showed that JC$\lambda$7 was transcriptionally expressed by four independent analyses.In a separate set of studies, other genes, called $\lambda$-like genes, were characterized and analyzed for expression in pre-B and B cell tissues. These gene products may be involved in pre-B cell development. We found a new $\lambda$-like gene, 16.2, in addition to the $\lambda$-like genes 14.1 and 16.1 previously described. To determine the transcription of all three $\lambda$-like genes in various pre-B cell sources, we used specific restriction enzyme digests of cDNA PCR products to distinguish these genes. Pre-B cell lines (ALLs, acute lymphocytic leukemias) were found to transcribe only gene 14.1. In contrast, fetal liver hybridomas were found to transcribe only gene 16.2. Examination of fetal liver tissue revealed the presence of both gene 14.1 and 16.2 transcripts, but a total lack of gene 16.1 transcripts.The use of somatic cell hybrid panels and pulse-field gel electrophoresis revealed the $\lambda$-like genes are grater than 700 kb distal to the JC$\lambda$ locus on chromosome 22q11.2, and 14.1 and 16.2 genes reside within a 210 kb restriction fragment, and gene 16.1 is at least 200 kb distal to genes 14.1 and 16.2.In conclusion, we found the human immunoglobulin lambda gene locus contains a fourth functional gene, JC$\lambda$7, in addition to JC$\lambda$1, 2, 3 and that two lambda related genes, 14.1 and 16.2, are transcribed in pre-B cells.

From Our News & Events

Deep Dive explores ‘Reefs, Wrecks, and Rascals’

Arthur Dunkelman, curator of the University of Miami Kislak Collection, shared his passion and expertise in a webinar...
Honoring the founding women of the Cuban Heritage Collection

Without their foresight and love of homeland, the collection—the largest repository of Cuban documents outside of the...
Celebrating ‘One Hundred Years of Solitude’

UM Libraries is presenting an extraordinary exhibit that immerses the audience in an emotional journey through the novel.
Cuban Heritage Collection to host book talk on the life in exile of Cuba's 5th president

Join us August 11 for a conversation, Q&A, and signing with author Francisco Santeiro and historian Daniel Pedreira.
University of Miami Libraries system offers a plethora of goods, services

In addition to safe study spaces, consultations, tutoring, remote access to computers, use of laptops, and access to video...
The power of books

A former UM professor started a company dedicated to publishing books on African-American culture, helping to give students...
Statement of Commitment

University of Miami Libraries denounces recent deaths, condemns violence and racism
The emotional potency of poetry

With the launch of National Poetry Month, University of Miami faculty members celebrate the solace and salve that poetry...
In search of hidden worlds

Every other Sunday evening, the Foote University Greene is transformed into a makeshift observatory by a dedicated group...
A gem of an art show

No longer “on line,” the Beaux Arts Festival on the UM campus this weekend, rated among the top fairs in the country,...

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From Our Digital Collections

From Our News & Events

Deep Dive explores ‘Reefs, Wrecks, and Rascals’

Honoring the founding women of the Cuban Heritage Collection

Theses & Dissertations (Scholarship@Miami)

Human immunoglobulin lambda light chain genes and lambda-like genes: Identification and characterization of a new C lambda gene and chromosomal localization and expression of human lambda-like genes in pre-B lymphocytes

From Our News & Events

Deep Dive explores ‘Reefs, Wrecks, and Rascals’

Honoring the founding women of the Cuban Heritage Collection

Celebrating ‘One Hundred Years of Solitude’

Cuban Heritage Collection to host book talk on the life in exile of Cuba's 5th president

University of Miami Libraries system offers a plethora of goods, services

The power of books

Statement of Commitment

The emotional potency of poetry

In search of hidden worlds

A gem of an art show

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